Peripheral blood cell preparation influences the level of expression of leukocyte cell surface markers as assessed with quantitative multicolor flow cytometry
نویسندگان
چکیده
منابع مشابه
Multicolor analysis of cell surface marker of human leukemia cell lines using flow cytometry.
BACKGROUND Leukemia cell lines are utilized as tools for molecular analysis. Their implementation in therapy will require standards for quality control, including appropriate selection criteria for functional analysis and efficacy determination. MATERIALS AND METHODS Characteristics of six human leukemia cell lines -Kasumi-1, NB-4, MOLM-13, MV-4-11, K562, and Jurkat cells-were investigated us...
متن کاملToward quantitative fluorescence measurements with multicolor flow cytometry.
A procedure is presented for calibrating the output of a multicolor flow cytometer in units of antibodies bound per cell (ABC). The procedure involves two steps. First, each of the fluorescence channels of the flow cytometer is calibrated using Ultra Rainbow beads with assigned values of equivalent number of reference fluorophores (ERF). The objective of this step is to establish a linear relat...
متن کاملRole of resistance training with the approach of blood flow restriction in skeletal muscle cell growth
Background: The aim of this study was to investigate the response of ERK1/2 protein and muscular morphological adaptations to a period of resistance training with local blood flow restriction. Materials and methods: Twenty healthy male Wistar rats without clinically evident disease (5 weeks old, 120±7 g weight) were divided into four equal groups: control, control with limited blood flow...
متن کاملبررسی تعداد سلول‌های کشنده طبیعی و بیان HLA-G1 در خون محیطی خانم‌های باردار تهدید به سقط
Background: Pregnancy is a phenomenon that antigens of semi allogenic fetus are in direct contact with mother's immune system. Immune dysregulation can cause fetus rejection by mother's immune system responses. Human leukocyte antigen-G1, as an immunotolerant molecule has a major role to induce tolerance during pregnancy by suppression of natural killer cells through inhibitor receptors on thes...
متن کاملInvestigation of NK Cell Population in Peripheral Blood and Tumor Lesions of Patients with Breast Cancer
Background: Alteration in peripheral blood lymphocytes (PBLs) is usually investigated to provide an evidence of the host immune responses to tumor antigens. The tumor infiltrating NK cells interact most closely with the tumor cells and more accurately reflect tumor host interactions. Objective: To analyze peripheral blood and tumor associated Natural Killer (NK) cells in patients with breast ca...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cytometry
سال: 1995
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990220208